SAT Question 0d088ae0: Transitions answer and explanation

English - Transitions - Cause Effect Transition
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Observing that a fire in a closed container soon went out, leading eighteenth-century scientists did not conclude that fresh air (specifically, oxygen) is necessary for combustion; instead, many theorized that the container’s air had become saturated with a substance called phlogiston. ______ when Joseph Priestley first isolated oxygen gas in 1774, he termed it “dephlogisticated air.”
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Difficulty:Medium
Which choice completes the text with the most logical transition?