SAT Question 8f6d6ae6: Boundaries answer and explanation

English - Boundaries - Punctuating Nonessential Element
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Archaeologists have estimated that the pre-Columbian Native American city of Cahokia, located across the Mississippi River from modern-day St. Louis, Missouri, had as many as 20,000 inhabitants in the year 1150 ______ it one of the largest cities in North America at the time.
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Difficulty:Medium
Which choice completes the text so that it conforms to the conventions of Standard English?